Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Shi-de Lin
China
Research Article
Differential Proteomics Analysis of Oligodendrogliomas and Astrocytomas Using iTRAQ Quantification
Author(s): Shi-de Lin, Jing-zhe Li, Peng Wang, Yu-jie Zang and Ting-bao ZhaoShi-de Lin, Jing-zhe Li, Peng Wang, Yu-jie Zang and Ting-bao Zhao
Background: Astrocytoma and oligodendroglioma are two histological subtypes of primary Central Nervous System (CNS) tumors. Because of the high cytoarchitectural variability and lacking accurate diagnosis biomarkers, distinguishing astrocytomas and oligodendrogliomas clinically remains challenging. Methods: The total protein lysates from of astrocytoma and oligodendroglioma clinical specimens were analyzed by 2DLC/MS/MS and quantified via Isobaric Tags Relative and Absolute Quantitation (iTRAQ). Differentially expressed proteins were further analyzed by Ingenuity Pathway Analysis (IPA) software. Lastly, potential bio-markers’ expression levels were validated by western blot. Results: A total 1856 proteins were identified. Among them, 83 proteins were increased and 82 proteins were decreased in astroglioma specimens compared to oligodendroglioma. Our bioinformatics study showed t.. View More»
DOI:
10.4172/jpb.1000433