Journal of Glycobiology
Open Access

Shingo Nakamura

Publications

• Research Article
  Novel Cancer Vaccination System Based on Human Endo-Β-N-Acetyl Glucosaminidase Gene Delivery
  Author(s): Satoshi Watanabe, Seiki Haraguchi, Shingo Nakamura, Takayuki Sakurai, Shin-ichiro Mugikura, Kagemasa Kajiwara, Minoiru Kimura and Masahiro Sato Satoshi Watanabe, Seiki Haraguchi, Shingo Nakamura, Takayuki Sakurai, Shin-ichiro Mugikura, Kagemasa Kajiwara, Minoiru Kimura and Masahiro Sato
  
  Cancer vaccination elicits an immune response against specific glycans or proteins expressed on the cell surface after gene transfer has occurred. We previously demonstrated that N-acetylglucosamine (GlcNAc) residues exposed after digestion with endo-?-galactosidase, a carbohydrate-digesting enzyme, elicited this type of immune response, probably as a result of the presence of natural antibodies recognizing GlcNAc residues in host animals. Treatment of a cell with endo-?-N-acetylglucosaminidase (ENGase), an enzyme that cleaves the amide bond between the proximal GlcNAc residues at the side chain of an asparagine residue on N-glycans, also causes exposure of GlcNAc residues on the cell surface. In this study, we examined whether mouse melanoma B16 cells transfected with a human ENGase (hENGase) cDNA expression construct, are susceptible to an immune attack after subcutaneous grafting to t.. View More»
  DOI: 10.4172/2168-958X.1000106

  Abstract PDF

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