ISSN: 2329-8936
Shridhar Jambagi
United Kingdom
Research Article
Global Transcriptome Analysis and Identification of Differentially Expressed Genes after Infection of Fragaria vesca with Powdery Mildew (Podosphaera aphanis)
Author(s): Shridhar Jambagi,Jim M. DunwellShridhar Jambagi,Jim M. Dunwell
Background: Podosphaera aphanis, the causal agent of strawberry powdery mildew causes significant economic loss worldwide.
Methods: We used the diploid strawberry species Fragaria vesca as a model to study plant pathogen interactions. RNA-seq was employed to generate a transcriptome dataset from two accessions, F. vesca ssp. vesca Hawaii 4 (HW) and F. vesca f. semperflorens Yellow Wonder 5AF7 (YW) at 1 d (1 DAI) and 8 d (8 DAI) after infection.
Results: Of the total reads identified about 999 million (92%) mapped to the F. vesca genome. These transcripts were derived from a total of 23,470 and 23,464 genes in HW and YW, respectively from the three time points (control, 1 and 8 DAI). Analysis identified 1,567, 1,846 and 1,145 up-regulated genes between control and 1 DAI, control and 8 DAI, and 1 and 8 DAI, respectively .. View More»
DOI:
10.4172/2329-8936.1000106