Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
Shusheng Wang
New Orleans, LA, 70118
Tanzania
Research Article
miR-146a is Upregulated During Retinal Pigment Epithelium (RPE)/Choroid Aging in Mice and Represses IL-6 and VEGF-A Expression in RPE Cells
Author(s): Yi Hao, Qinbo Zhou, Jing Ma, Yun Zhao and Shusheng Wang
Yi Hao, Qinbo Zhou, Jing Ma, Yun Zhao and Shusheng Wang
Purpose: MicroRNA-146a (miR-146a) has been proposed as a marker for age-associated inflammation, or “inflammaging”, acting as a negative regulator of cellular senescence and pro-inflammatory signalling pathways. However, the regulation and function of miR-146 during ocular aging remains unclear. Here we propose that miR-146 is regulated during aging of the retina and choroid, and functions in retinal pigment epithelial (RPE) cells to regulate key genes involved in inflammation and angiogenesis.
Methods: The expression of miR-146a and miR-146b was examined in the neuroretina and RPE/choroid in mice aged from 2 months to 24 months. Then, the effect of synthetic miR-146a mimetic on IL-6 and VEGF-A expression was analysed in RPE cells treated with and without TNF-α.
Results: miR-146a and miR-146b were upreg.. View More»
DOI:
10.4172/2155-9570.1000562