Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Soile Tapio
Helmholtz Center Munich, German Research Center for Environmental Health, Institute of Radiation Biology, Neuherberg,
Germany
Research Article
Radiation-Induced Crosstalk between MicroRNAs and Proteins of the
Endothelium: In silico Analysis
Author(s): Arundhathi Sriharshan, Anne Kraemer, Michael J Atkinson, Simone Moertl and Soile TapioArundhathi Sriharshan, Anne Kraemer, Michael J Atkinson, Simone Moertl and Soile Tapio
Ionising radiation causes damage at various levels in the exposed cell. The initial injury and the resulting cellular response to the damage involve complex crosstalk between the regulators of the DNA damage response recognition signalling and repair pathways. System-level research is required to gain more insight into these pathways. In this study we have used an in silico method to connect the altered proteome and miRNAome networks after radiation exposure by using Ingenuity Pathway Analysis tool and further verification for seed sequence matches by manually searching in microrna.org, mirDB, mirwalk, miRBase, and Targetscan databases. The endothelial cell was used as a model system as the endothelium is one of the main cellular systems damaged by ionising radiation. The interaction analysis revealed that changes at the miRNA level occur shortly after irradiation (4 and 12 hours) and.. View More»
DOI:
10.4172/jpb.1000335