Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Soodeh Kianfar
West Indies
Research Article
Detection of Numerical Aneuploidy of Chromosomes X, Y, 13, 18 and 21 in 100 Blood and Fetals Samples by QF-PCR Method
Author(s): Sirous Zeinali, Zahra Kharazi Tavakol, Soodeh Kianfar, Ariana Kariminejad, Nejat Mahdieh, Mehrdad Hashemi and Zahra ZeinaliSirous Zeinali, Zahra Kharazi Tavakol, Soodeh Kianfar, Ariana Kariminejad, Nejat Mahdieh, Mehrdad Hashemi and Zahra Zeinali
Objective: Determination of parents to avoid giving birth to a child with any anomalies has increased the demand for prenatal diagnosis. Two most important criteria of any prenatal diagnosis procedure are accuracy, and speed. They have to minimize burden and anxiety for families. One of the main tests requested during pregnancies is testing for chromosomal abnormalities. This study was carried out with the aim of investigating the use of a rapid diagnosis test for detecting chromosomal numerical aneuploidy in blood and fetal samples, and also to compare the outcomes with cytogenetic method. Materials and methods: In this study, 100 samples from high risk pregnancies or affected individuals, comprising 12 chorionic villi (CV), 43 amniotic fluids (AF) and 45 blood samples were analyzed by QF-PCR. The samples were amplified using the specific microsatellite markers (STRs) for chromosomes.. View More»
DOI:
10.4172/jpb.1000227