Biochemistry & Pharmacology: Open Access
Open Access
ISSN: 2167-0501
+44-20-4587-4809
ISSN: 2167-0501
+44-20-4587-4809
Stefan U Weber
Department of Anesthesiology and Intensive Care Medicine, University Bonn Medical Center,
Sigmund-Freud-Str. 25, 53105 Bonn
Germany
Research Article
Statins Downregulate the Constitutive Expression of HLA-DR and Reduce Intracellular CD74 in the Monocyte Cell Line Mono Mac 6
Author(s): Stefan U Weber, Lutz E Lehmann, Makbule Kobilay, Frank Stüber and Andreas HoeftStefan U Weber, Lutz E Lehmann, Makbule Kobilay, Frank Stüber and Andreas Hoeft
Background: Statins exert immune effects and have been shown to strongly inhibit the interferon-γ induced expression of the human leukocyte antigen (HLA) DR. Monocyte HLA-DR is decreased severe in sepsis and indicates hypo inflammatory and immunosuppressive phases. The aim of the study was to investigate the statin-induced regulation of constitutive HLA-DR expression on monocytes.
Method: Monocytes and the monocyte cell line Mono Mac 6 were incubated with simvastatin, mevastatin, pravastatin and fluvastatin. HMG-Coenzyme A reductase was inhibited by l-mevalonate. Protein expression of HLA-DR, Major histocompatibility complex, invariant CD74 and apoptosis were measured by flow-cytometry. mRNA expression was assessed by PCR.
Results: Simvastatin dose dependently reduced the constitutive HLA-DR expression on monocytes starting from 500 n.. View More»
DOI:
10.4172/2167-0501.1000200