Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Sture Forsgren
Anatomy Section, Umeå University,
SE-901 87 Umeå
Sweden
Research Article
TNF-alpha in an Overuse Muscle Model-Relationship to Muscle Fiber Necrosis/Regeneration, the NK-1 Receptor and an Occurrence of Bilateral Involvement
Author(s): Lina Renström, Yafeng Song, Per S Stål and Sture ForsgrenLina Renström, Yafeng Song, Per S Stål and Sture Forsgren
TNF-alpha is known to be involved in muscle damage and inflammation (myositis). The relationships between the TNF-alpha system and muscle fiber necrosis/regeneration and the tachykinin system in this situation are unclear. We have an experimental rabbit model related to unilateral muscle overuse which leads to marked muscle derangement and myositis bilaterally. Using this model, staining for TNF-alpha, in parallel with staining for the substance P-preferred receptor (NK-1R) and desmin were performed. Desmin staining was used as a reference concerning identification of degeneration/regeneration and the soleus muscle was the muscle examined. It was observed that the inflammatory cells, as well as blood vessel walls in the myositis areas, expressed TNF-alpha mRNA. Muscle fibers that were interpreted to represent necrotic fibers expressed TNF-alpha mRNA reactions and showed NK-1R immunore.. View More»
DOI:
10.4172/2155-9899.1000138