Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
Sujata Prasad
India
Research Article
Biofilm: The Haven for Staphylococcus epidermidis in Post-operative Endophthalmitis
Author(s): Sujata Prasad, Niranjan Nayak, Gita Satpathy, Tapas Chandra Nag, Pradeep Venkatesh and Ravinder Mohan PandeySujata Prasad, Niranjan Nayak, Gita Satpathy, Tapas Chandra Nag, Pradeep Venkatesh and Ravinder Mohan Pandey
Background: Staphylococcus epidermidis, in spite of being a commensal of the eye, is regarded as one of the common etiological agents of bacterial endophthalmitis. The purpose of the study was to determine the pathogenic potential of this commensal organism in post-operative endophthalmitis. Methods: A total of 63 S epidermidids isolates, comprising of 47 from vitreous samples of post-operative endophthalmitis cases and 16 controls from the healthy conjunctivae, were studied. Biofilm producing ability was determined by quantitative adherence test and "ica AB" Polymerase Chain Reaction (PCR) assay. Bacteria adhering onto the lens surfaces were enumerated by standard protocol. Scanning electron microscopy (SEM) was employed to visualize the biofilms on the lenses. Results: Of the 47 isolates, 23 (48.9%) wer.. View More»
DOI:
10.4172/2155-9570.1000350