ISSN: 2329-9029
+44 1478 350008
Suresh Chandra
India
Research Article
Efficient In Vitro Regeneration, Analysis of Molecular Fidelity and Agrobacterium tumifaciens - Mediated Genetic Transformation of Grewia asiatica L.
Author(s): Wani TA, Satiander Rana, Bhat WW, Pandith SA, Niha Dhar, Sumeer Razdan, Suresh Chandra, Namrata Sharma and Surrinder K LattooWani TA, Satiander Rana, Bhat WW, Pandith SA, Niha Dhar, Sumeer Razdan, Suresh Chandra, Namrata Sharma and Surrinder K Lattoo
Grewia asiatica is a dietotheraphtically important fruit bearing shrub, indigenous to India. It is a rich resource of triterpinoids and flavonoids and possesses many putative health benefits. Two of the drawbacks which include short shelf life of its fruits and larger seed volume impedes its full exploitation. Seed abortion for developing seedless cultivars through biotechnological interventions is a viable option. One of the prerequisites for such strategy is to develop an efficient plant regeneration and transformation protocols in G. asiatica. Against this backdrop multiple shoot induction was achieved from nodal explants with axillary buds, on culturing in Woody Plant medium (WM) fortified with 3% (w/v) sucrose, 2 × 10-5M Kinetin (Kn) and 1 × 10-5M indole-3-butyric acid (IBA) giving rise to an average of 4.25 ± 0.71 microshoots per explant. More than 90% of the .. View More»
DOI:
10.4172/2329-9029.1000167