Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Tadashi Kondo
National Cancer Center Research Institute,
5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045
Japan
Research Article
The Proteomic Profile of Pancreatic Cancer Cell Lines Corresponding to Carcinogenesis and Metastasis
Author(s): Masayo Yamada, Kiyonaga Fujii, Koji Koyama, Setsuo Hirohashi and Tadashi Kondo
Masayo Yamada, Kiyonaga Fujii, Koji Koyama, Setsuo Hirohashi and Tadashi Kondo
To investigate the proteomic background of the carcinogenesis and progression of pancreatic cancer, the protein expression profiles of nine well-characterized pancreatic adenocarcinoma cell lines, whose metastatic potential was previously examined in a mouse xenograft model, and two immortalized pancreatic duct cell lines were examined. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 126 protein spots the intensity of which was significantly different between the normal pancreatic duct cell lines and the pancreatic cancer cell lines with different metastatic potential. Mass spectrometric protein identification demonstrated that these protein spots corresponded to 95 unique genes, which included proteins not previously shown to be aberrant in pancreatic cancer. To characterize the observed proteome, LC-MS/MS identified the proteins corresponding to .. View More»
DOI:
10.4172/jpb.1000057