Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Tadashi Kondo
National Cancer Center Research Institute,
5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045
Japan
Research Article
Proteomic Profile of Epithelioid Sarcoma
Author(s): Kenta Mukaihara, Daisuke Kubota, Akihiko Yoshida, Naofumi Asano, Yoshiyuki Suehara, Kazuo Kaneko, Akira Kawai and Tadashi KondoKenta Mukaihara, Daisuke Kubota, Akihiko Yoshida, Naofumi Asano, Yoshiyuki Suehara, Kazuo Kaneko, Akira Kawai and Tadashi Kondo
Epithelioid sarcoma (ES) is a rare soft tissue sarcoma affecting young adults. It is a slow-growing tumor with a high rate of recurrence and metastasis to lymph nodes. Although deletion of the tumor suppressor gene, SMARCB1/ INI1, has been identified in ES, the molecular background factors are largely unknown. To clarify the molecular aberrations contributing to the malignant features of ES, we investigated the proteins present in ES tumor tissues. Two-dimensional difference gel electrophoresis of homogenized tissue samples revealed 3363 protein spots, of which 91 showed differences in intensity between tumor and adjacent non-tumor tissues in eight ES cases. Using mass spectrometry, we characterized 69 unique proteins corresponding to these protein spots. We found that the complex histology of ES was obstacle for the investigation of molecular backgrounds of ES. For instance, although.. View More»
DOI:
10.4172/jpb.1000316