Translational Medicine
Open Access

Teri A Longacre

Publications

• Research Article
  Quantitative and Sensitive Detection of Cancer Genome Amplifications from Formalin Fixed Paraffin Embedded Tumors with Droplet Digital PCR
  Author(s): Lincoln Nadauld, John F Regan, Laura Miotke, Reet K Pai, Teri A Longacre, Shirley S Kwok, Serge Saxonov, James M Ford1 and Hanlee P Ji* Lincoln Nadauld, John F Regan, Laura Miotke, Reet K Pai, Teri A Longacre, Shirley S Kwok, Serge Saxonov, James M Ford1 and Hanlee P Ji*
  
  For the analysis of cancer, there is great interest in rapid and accurate detection of cancer genome amplifications containing oncogenes that are potential therapeutic targets. The vast majority of cancer tissue samples are formalin fixed and paraffin embedded (FFPE) which enables histopathological examination and long term archiving. However, FFPE cancer genomic DNA is often degraded and generally a poor substrate for many molecular biology assays. To overcome the issues of poor DNA quality from FFPE samples and detect oncogenic copy number amplifications with high accuracy and sensitivity, we developed a novel approach. Our assay requires nanogram amounts of genomic DNA, thus facilitating study of small amounts of clinical samples. using droplet digital PCR (ddPCR), we can determine the relative copy number of specific genomic loci even in the pres.. View More»
  DOI: 10.4172/2161-1025.1000107

  Abstract PDF

Relevant Topics