Journal of Cell Science & Therapy
Open Access

Tomasz Oldak

Publications

• Research Article
  Comparison of Characteristics of Mesenchymal Stem Cells Obtained Mechanically and Enzymatically from Placenta and Umbilical Cord
  Author(s): Ekaterina Semenova, Eugeniusz K Machaj and Tomasz Oldak Ekaterina Semenova, Eugeniusz K Machaj and Tomasz Oldak
  
  Mesenchymal stem cells (MSC) are a good source for the cell therapy thanks to their abilities. MSCs exert very important immunomodulatory effects: they suppress T- and B-cell proliferation and natural killer cell function, and they also limit the expression of the Major Histocompatibility Complex II. Placenta and umbilical cord are the most convenient sources of MSCs, which do not entail ethical problems. Additionally, placental delivery and isolation of umbilical cord is not connected with a difficult or invasive method. MSCs from Wharton’s jelly and placenta (the amnion, the chorion, the villi, the Decidua basalis) were isolated with the protocol: mechanically and enzymatically (collagenase digestion). The proliferation potential was evaluated with a PDT analysis. For multi-differentiation of MSCs, cells were incubated in different differentiation media for 2-3 weeks at 37°.. View More»
  DOI: 10.4172/2157-7013.1000262

  Abstract PDF

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