ISSN: 2167-0412
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Ubaid Yaqoob
Plant Molecular Biology Lab, International Centre for Genetic Engineering and Biotechnology,
Aruna Asaf Ali Marg, New Delhi-110 067
India
Research Article
In-Silico Analysis, Structural Modelling and Phylogenetic Analysis of
Acetohydroxyacid Synthase Gene of Oryza sativa
Author(s): Ubaid Yaqoob, Tanushri Kaul and Irshad Ahmad Nawchoo
Ubaid Yaqoob, Tanushri Kaul and Irshad Ahmad Nawchoo
The Acetohydroxyacid synthase (EC 2.2.1.6) or Acetolactate synthase (ALS) belongs to a family of thiamine diphosphate (TPP) dependent enzymes which catalyzes the first reaction in the biosynthesis of essential amino acids - isoleucine, leucine and valine. Acetohydroxyacid synthase (AHAS) is present in plants, algae, fungi and bacteria and is found to be a vital target of multiple herbicides. We revealed the homology model of OsAHAS protein using the structure of Arabidopsis thaliana AHAS (PDB ID: 3E9Y) as template. The resulting model structure was refined by PROCHECK, ProSA, RMSD and Verify3D that indicated the model structure is reliable with 76% amino acid sequence identity with template. RMSD (1.75Å), Verify3D (86.02%), Z-score (-9.55) and Ramachandran plot analysis showed that conformations for 81.6% of amino acid residues are within the most favoured regions. The .. View More»
DOI:
10.4172/2167-0412.1000272