Transcriptomics: Open Access
Open Access

Ulises D Orlando

Publications

• Research Article
  Gene Expression Profile and Signaling Pathways in MCF-7 Breast Cancer Cells Mediated by Acyl-Coa Synthetase 4 Overexpression
  Author(s): Ana F Castillo, Ulises D Orlando, Paula Lopez, Angela R Solano, Paula M. Maloberti and Ernesto J Podesta Ana F Castillo, Ulises D Orlando, Paula Lopez, Angela R Solano, Paula M. Maloberti and Ernesto J Podesta
  
  Aim: Breast cancer comprises a heterogeneous group of diseases that vary in morphology, biology, behavior and response to therapy. Previous studies have identified an acyl-CoA synthetase 4 (ACSL4) gene-expression pattern correlated with very aggressive tumors. In particular, we have used the tetracycline Tet-Off system to stably transfect non-aggressive breast cancer MCF-7 cells and developed a stable line overexpressing ACSL4 (MCF-7 Tet-Off/ACSL4). As a result, we have proven that cell transfection solely with ACSL4 cDNA renders a highly aggressive phenotype in vitro and results in the development of growing tumors when injected into nude mice. Nevertheless, and in spite of widespread consensus on the role of ACSL4 in mediating an aggressive phenotype in breast cancer, the early steps through which ACSL4 increases tumor growth and progression have been scarcely descr.. View More»
  DOI: 10.4172/2329-8936.1000120

  Abstract PDF

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