ISSN: 2375-4508
+44 1478 350008
Upekha Basnayaka
Canada
Research Article
Synchrotron Imaging of Ovaries Ex Vivo
Author(s): Upekha Basnayaka, Dean Chapman, Gregg Adams, Tomasz Wysokinski, George Belev, Rani Kanthan, Rajni Chibbar, Robert Lewis, Naoto Yagi, Kentaro Uesugi, Masato Hoshino and Angela Baerwald
Upekha Basnayaka, Dean Chapman, Gregg Adams, Tomasz Wysokinski, George Belev, Rani Kanthan, Rajni Chibbar, Robert Lewis, Naoto Yagi, Kentaro Uesugi, Masato Hoshino and Angela Baerwald
Background: Conventional 2-dimensional ultrasonography is limited in its ability to detect ovarian microanatomy. The objective of this study was to determine if biomedical synchrotron techniques would be effective for imaging ovarian microanatomy, including ovarian follicles, corpora lutea, and the oocyte.
Methods: A prospective, observational study was conducted at the Canadian Light Source to compare ovarian imaging ex vivo using propagation-based computed tomography (PB-CT) synchrotron imaging, ultrasonography, and histology. Bovine (n=4) and human (n=4) ovaries were imaged fresh or formalin-fixed. The effectiveness of Talbot grating interferometry computed tomography (TGI-CT) synchrotron imaging to image preserved bovine (n=1) and human (n=1) ovaries were evaluated at the SPring-8 synchrotron, Japan.
Results: A.. View More»
DOI:
10.4172/2375-4508.1000169