Lupus: Open Access
Open Access

Valentina N. Buneva

Publications

• Research Article
  SLE: Unusual Recombinant Monoclonal Light Chain NGTA3-Pro-DNase Possessing Three Different Activities Trypsin-like, Metalloprotease and DNase
  Author(s): Anna M. Timofeeva, Valentina N. Buneva and Georgy A. Nevinsky*Anna M. Timofeeva, Valentina N. Buneva and Georgy A. Nevinsky*
  
  Objective: DNA- and myelin basic protein (MBP)-hydrolyzing antibodies can play an important role in the pathogenesis of systemic lupus erythematosus (SLE), therefore their analysis seems to be important. Results: In mammalians there are serine proteases, metalloproteases, and DNases. Each of these and many other enzymes catalyze only one chemical reaction. Very unusual and unpredictable situation was revealed by us in the case of monoclonal abzymes corresponding to the sera of patients with SLE. The small pools of phage particles displaying light chains with different affinity for myelin basic protein (MBP) were isolated by affinity chromatography on MBP-Sepharose. In contrast to canonical enzymes, one of twenty five MLChs demonstrated three different enzymatic activities; it efficiently hydrolyzed MBP (but not other proteins) and DNA. Other.. View More»
  DOI: 10.35248/2684-1630.17.2.127

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