International Journal of Physical Medicine & Rehabilitation
Open Access
ISSN: 2329-9096
+44 1300 500008
ISSN: 2329-9096
+44 1300 500008
Valesca Veiga Cardoso
Department of Mutagenesis and Toxicology, Centro Universitário Metodista,
Avenue Demetrio Ribeiro, 151 apt 702 Porto Alegre-RS
Brazil
Research Article
Evaluation of Mutagenic Effects of Chronic Renal Disease and Hemodialysis
Author(s): Evânio Márcio Romanzini, Caroline Dani, Jussiene Magnus Justos, Kimberly Rosa Martins, Marcello Ávilla Mascarenhas and Valesca Veiga CardosoEvânio Márcio Romanzini, Caroline Dani, Jussiene Magnus Justos, Kimberly Rosa Martins, Marcello Ávilla Mascarenhas and Valesca Veiga Cardoso
The present study aimed to evaluate the mutagenic effects of chronic renal disease (CRD) and haemodialysis (HD) using the mutagenic test known as the micronucleus assay The study involved 97 individuals, including 32 CRD patients on HD, 34 CRD patients not on HD and 31 controls. Buccal smears were examined with a light microscope at 400, and cells showing micronuclei, binucleated nuclei, “broken egg” nuclei, pyknosis, karyorrhexis and karyolysis were counted in 2000 cells for each participant. Our main results demonstrate that the clastogenicity indicators micronuclei, binucleated cells, broken egg cells, sum of binucleated plus broken egg cells and sum of pyknosis plus karyorrhexis were statistically significant higher in the CRD and HD group compared to the CRD and non-HD groups and the control group (p=0.0001). Males had more binucleated cells (p=0.002) and sum of binuclea.. View More»
DOI:
10.4172/2329-9096.1000467