Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access
ISSN: 2375-4508
+44 1478 350008
ISSN: 2375-4508
+44 1478 350008
Vanden Berghe W
Belgium
Research Article
Investigation of Adverse Cancer Related DNA Methylation Variation in the Human Telomerase Reverse Transcriptase (hTERT) Promotor in Follicular Fluid Cells of Women Subjected to Ovarian Stimulation for In Vitro Fertilization (IVF)
Author(s): Comhaire FH, Decleer WAE, Declerck K and Vanden Berghe W
Comhaire FH, Decleer WAE, Declerck K and Vanden Berghe W
Purpose: To determine the variability in DNA methylation of the human telomerase reverse transcriptase (hTERT) promotor in cells of follicular fluid of patients receiving ovarian stimulation for treatment with in vitro fertilization (IVF), in connection with possible cancer related health risk in offspring.
Materials and method: The pilot study analysed the variability in DNA methylation of five cancer related CpG biomarkers in the hTERT promoter by bisulfite pyrosequencing in cells of follicular fluid of 8 patients treated by IVF, each one originating from one ovary.
Results: The absolute promoter methylation levels of individual CpG dinucleotides in the hTERT promoter region in cells of follicular fluid of patients undergoing ovarian stimulation for IVF varied between 2 and 15%. Although the mean hTERT promoter m.. View More»
DOI:
10.4172/2375-4508.1000161