Journal of Proteomics & Bioinformatics
Open Access

Varun Gopala Krishna

Publications

• Research Article
  Broad Utility of an Affinity-enrichment Strategy for Unanchored Polyubiquitin Chains
  Author(s): Jo Strachan, Barry Shaw, Varun Gopala Krishna, Daniel Scott, Juan C del Pozo, Kristine Hill, Madhav Gautam, Dorota Skowyra, Andrew D. Jacobson, Chang-Wei Liu, Neil J Oldham and Rob LayfieldJo Strachan, Barry Shaw, Varun Gopala Krishna, Daniel Scott, Juan C del Pozo, Kristine Hill, Madhav Gautam, Dorota Skowyra, Andrew D. Jacobson, Chang-Wei Liu, Neil J Oldham and Rob Layfield
  
  Protein ubiquitination is a common post-translational modification where selected targets are covalently modified by the ubiquitin protein, often in the form of isopeptide-linked polyubiquitin chains. More recently, unanchored (i.e. non-substrate-linked) polyubiquitin chains have also been described and implicated in a range of biological processes. The development of Tandem-repeated Ubiquitin-Binding Entities (TUBEs), engineered repeats of ubiquitin-binding domains that interact non-covalently with polyubiquitin, has allowed strategies for the affinity-enrichment of ubiquitinmodified proteins to be established, in some cases with linkage specificity. Here, we demonstrate the utility of a Free Ubiquitin-Binding Entity (FUBE), based on an ubiquitin-binding domain with high specificity for the free C-terminus of ubiquitin (the Znf-UBP domain of human USP5). In contrast to TUBEs which do.. View More»
  DOI: 10.4172/jpb.S7-001

  Abstract PDF

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