Andrology-Open Access
Open Access

Vijay Prabha

Publications

• Research Article
  Amelioration of Sperm Agglutinating Factor (SAF) Induced Sperm Impairment by Anti-SAF Polyclonal Antibody
  Author(s): Kiranjeet Kaur, Praveen Rishi and Vijay Prabha Kiranjeet Kaur, Praveen Rishi and Vijay Prabha
  
  Background: We have previously isolated a Spermagglutinating Factor (SAF) from Escherichia coli, which was capable of causing sperm agglutination and impairment of sperm parameters viz. apoptosis, premature acrosome loss and inhibition of Mg2+ dependent ATPase activity invitro. In addition, intravaginal administration of Balb/c mice with SAF resulted in infertility. To provide evidence that SAF plays an important role in impairment of sperm parameters and infertility, anti-SAF antiserum was raised and its application as a therapeutic intervention against SAF induced damage was evaluated. Methods: Effect of anti-SAF antiserum was evaluated against SAF mediated adverse effects on sperm parameters. Sperm agglutination was observed using light microscopy and Mg2+ dependent ATPase activity was estimated in terms of release of inorganic phosphate. Sperm apoptosis and acrosome stat.. View More»
  DOI: 10.4172/2167-0250.1000109

  Abstract PDF

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