Journal of Physical Chemistry & Biophysics
Open Access

Vyatchin IG

Publications

• Research Article
  Natural Actin and Tropomyosin from Molluscan Catch Muscle
  Author(s): Girich UV, Lazarev SS, Vyatchin IG, Matusovsky OS and Sheludko NS* Girich UV, Lazarev SS, Vyatchin IG, Matusovsky OS and Sheludko NS*
  
  In this work, the main proteins of thin filaments (actin and tropomyosin) were isolated from the catch muscle of the mussel Crenomytilus grayanus and the rabbit skeletal muscles. Rabbit actin and tropomyosin and mussel tropomyosin were isolated by traditional methods as opposed to "natural" mussel actin (isolation of the mussel actin from acetone powder is impossible). These proteins were used to reconstruct actin+tropomyosin complexes in nonhybrid and hybrid manner. A non-hybrid complex, reconstructed from rabbit actin and tropomyosin, has higher reduced viscosity than complex of mussel proteins where reduced viscosity was nearly indistinguishable from the intrinsic viscosity. ÐÂ’oth rabbit and mussel actin were purified by polymerization-repolymerization cycles followed by gel filtration chromatography. During purification, the viscosity of both ac.. View More»
  DOI: 10.4172/2161-0398.1000249

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