Cell & Developmental Biology
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Weisburg JH

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• Research Article
  Selective Cytotoxicity of a Grape Seed Proanthocyanidin Extract to Human Oral Carcinoma HSC-2 Cells
  Author(s): Alyssa G. Schuck, Weisburg JH, Greenbaum RE, Golfeiz MD, Segal JR, Weiss RA, Liebman EC, Zuckerbraun HL and Babich H Alyssa G. Schuck, Weisburg JH, Greenbaum RE, Golfeiz MD, Segal JR, Weiss RA, Liebman EC, Zuckerbraun HL and Babich H
  
  Grape seed extract (GSE), a nutraceutical rich in polyphenol proanthocyanidins, was evaluated for its toxicity to human oral cells. Using the neutral red cytotoxicity assay, human squamous carcinoma (HSC-2) cells were shown to be more sensitive to GSE than were gingival fibroblasts. Polyphenols undergo auto-oxidation reactions in cell culture medium, to generate reactive oxygen species, in particular, hydrogen peroxide. Using the FOX assay to quantify hydrogen peroxide, GSE, when added to cell culture medium, generated hydrogen peroxide, albeit at relatively low levels. No hydrogen peroxide was detected in the presence of catalase, which catalyzes the decomposition of hydrogen peroxide, and minor levels were detected in the presence of superoxide dismutase, which stabilizes polyphenols. Superoxide free radical, detected with nitroblue tetrazolium, was identified in GSE-amended medium... View More»
  DOI: 10.4172/2168-9296.1000121

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