Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
William Hancock
Tanzania
Research Article
Proteomic Analysis of Bioreactor Cultures of an Antibody Expressing CHOGS
Cell Line that Promotes High Productivity
Author(s): Haimanti Dorai, Suli Liu, Xiang Yao, Yonghui Wang, Uelke Tekindemir, Michael J Lewis, Shiaw-Lin Wu and William Hancock
Haimanti Dorai, Suli Liu, Xiang Yao, Yonghui Wang, Uelke Tekindemir, Michael J Lewis, Shiaw-Lin Wu and William Hancock
Antibody manufacturing cell line development at Janssen Research & Development involves transfection of therapeutic antibody genes into a CHO-GS host cell line and isolating primary transfectomas that upon cloning yield high expressing cell lines secreting the desired antibody products. Subsequently, these cell lines are cultivated in stirred tank bioreactors for the large-scale generation of the products. In an attempt to optimize this process for high productivity, a two pronged approach was undertaken.
First, in a Design of Experiment study, a CHO-GS cell line expressing a therapeutic antibody was cultivated in 2 L DasGip fed-batch mini-bioreactors under a variety of culture conditions. In general, culture conditions that promoted robust growth and high viable cell density resulted in high productivity. Then, cell culture harvests and cell lysates fro.. View More»
DOI:
10.4172/jpb.1000268