Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
William M. McLaughlin
Tanzania
Research Article
Constitutive and Stress-induced Expression of CCL5 Machinery in Rodent Retina
Author(s): D’Anne S. Duncan, William M. McLaughlin, Noah Vasilakes, Franklin D. Echevarria, Cathryn R. Formichella and Rebecca M. SappingtonD’Anne S. Duncan, William M. McLaughlin, Noah Vasilakes, Franklin D. Echevarria, Cathryn R. Formichella and Rebecca M. Sappington
Signaling by inflammatory cytokines and chemokines is associated with neurodegeneration in disease and injury. Here we examined expression of the β-chemokine CCL5 and its receptors in the mouse retina and evaluated its relevance in glaucoma, a common optic neuropathy associated with sensitivity to intraocular pressure (IOP). Using quantitative PCR, fluorescent in situ hybridization, immunohistochemistry and quantitative image analysis, we found CCL5 mRNA and protein was constitutively expressed in the inner retina and synaptic layers. CCL5 appeared to associate with Müller cells and RGCs as well as synaptic connections between horizontal cells and bipolar cells in the OPL and amacrine cells, bipolar cells and RGCs in the IPL. Although all three high-affinity receptors (CCR5, CCR3, CCR1) for CCL5 were expressed constitutively, CCR5 expression was significantly higher.. View More»
DOI:
10.4172/2155-9899.1000506