ISSN: 0974-276X
+44 1223 790975
Wuying Chu
China
Research Article
Characterization and Expression Analysis of Myogenin Gene in White
Muscle of Chinese Mandarin Fish, Siniperca chuatsi
Author(s): Wuying Chu, Yulong Li, Ping Wu, Dunxue Chen, Jia Chen, Jun Shi and Jianshe Zhang
Wuying Chu, Yulong Li, Ping Wu, Dunxue Chen, Jia Chen, Jun Shi and Jianshe Zhang
Myogenin plays a crucial role in myoblast differentiation and maturation. In the present study, the myogenin gene structure and expressional patterns in Siniperca chuatsi were characterized. Sequence analysis indicated that the myogenin shared a similar structure and the conserved bHLH domain with other vertebrate myogenin genes. Sequence alignment and phylogenetic analysis showed that Siniperca chuatsi myogenin shared homologous with Epinephelus coioides, Sparus aurata, Takifugu rubripes and Salmo salar. Whole-mount in situ hybridization revealed that myogenin expression was first detected in the gastrula stage embryos and high levels of expression at the 24 somite stage. After the 24 somite stage, myogenin expression began to decrease in the anterior somites where somatic cells were diffe.. View More»
DOI:
10.4172/jpb.1000304