Translational Medicine

Translational Medicine
Open Access

ISSN: 2161-1025

Xiang Z

Xiang Z

Tanzania

Publications
  • Research Article
    Fluorescent Determination of Secretory Phospholipase A2 (sPLA2)-Mediated Human Serum Albumin Binding Activity with Membrane Phospholipids and Fatty Acids
    Author(s):  Tsao FHC, Xiang Z and Meyer KC  Tsao FHC, Xiang Z and Meyer KC

    Human serum albumin (HSA) is a complex protein with multiple functions and plays a key role in organ system homeostasis. Changes in albumin levels are associated with worsened outcome in critical illness. However, serum albumin functional activities cannot be easily determined to assess its capacities in illness. Two real-time and sensitive fluorescent liposome assays were developed to determine HSA binding activity, one for measuring the interaction activity between albumin and membrane phospholipids (PL), and one for measuring binding of albumin to fatty acid (FA). Secretory phospholipase A2 was used as a mediator in the assay. The sensitive, yet simple fluorescent assays were specific for measuring the activities of serum albumin binding with PL and FA. Among heterogeneous forms of albumin, a small specific fraction of albumin (SFA) was the only portion that interacted with membran.. View More»
    DOI: 10.4172/2161-1025.1000150

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