Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Xiaohong Qian
China
Research Article
Profiling of Phosphorylated Proteins in Human Fetal Liver
Author(s): Ying Jiang, Quanjun Wang, Jinglan Wang, Songfeng Wu, Gang Cheng, Handong Wei, Yunping Zhu, Xiaohong Qian and Fuchu He
Ying Jiang, Quanjun Wang, Jinglan Wang, Songfeng Wu, Gang Cheng, Handong Wei, Yunping Zhu, Xiaohong Qian and Fuchu He
Constitutively phosphorylated proteins in Human fetal liver (HFL) aged 16-24 wk of gestation were studied using a 2-DE step followed by western blotting detection and MS identification. We found 166 phosphorylated protein spots with quantitative information and identified 101 gene products. Of theses identified proteins, 57 contain phosphoserine, 49 contain phosphothreonine, 51 contain phosphotyrosine, and 64 were newly identified phosphorylated proteins. The possible phosphorylation sites were further predicted using Netphos, ScanProsite and Scansite programs and most proteins were predicted the same site by at least 2 programs. Integrating the functional categories, protein abundance and the degree of phosphorylation of these proteins, we found proteins related to carbohydrate, lipid and amino acid metabolism were highly expressed with also the high degree of all se.. View More»
DOI:
10.4172/jpb.1000052