Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Xingang Liu
IR Iran
Research Article
Simultaneous Determination of Six Insecticides in Okra (Abelmoschus esculentus L. Moench) by UHPLC-MS/MS
Author(s): Husham NM Hussan, Xingang Liu, Fengshou Dong, Jun Xu and Yongquan ZhengHusham NM Hussan, Xingang Liu, Fengshou Dong, Jun Xu and Yongquan Zheng
The sensitive analytical method using quick, easy, cheap, effective, rugged and safe (QuEChERS) method for simultaneous determination of six insecticides in Okra was developed by UHPLC -MS-MS. The six insecticides were extracted from Okra matrices using acetonitrile and subsequently clean up using only octadecylsilance as sorbent prior to LC-MS-MS analysis. The determination of the target compounds were achieved in less than 2.0 min using an electrospray ionization source in positive mode (ESI+) for imidacloprid, acetamiprid, thiacloprid, thiamethoxam, clothianidin and negative mode (ESI-) for flonicamid. The method showed that the limits quantification (LOQ) ranged from 0.13 to 5.9 μgkg-1 in all matrices. The matrix-matched standard gave satisfactory recoveries (72.4%-105.1%) and relative standard deviation (2.2 - 20.0%) values in different matrices at three spiked leve.. View More»
DOI:
10.4172/2157-7064.1000370