Journal of Leukemia
Open Access

Yajuan Zheng

Publications

• Research Article
  The Time-Dependent Morphological Alteration and Enucleation Process during the Differentiation of Mammalian Erythroid Cells
  Author(s): Zuli Yang, Yajuan Zheng, Fan Hu, Zhang Zhang, Fukun Zhao and Shifu Zhang Zuli Yang, Yajuan Zheng, Fan Hu, Zhang Zhang, Fukun Zhao and Shifu Zhang
  
  One characteristic of the erythroid terminal differentiation is hemoglobin expression and enucleation. In the present study, by using real-time cultured Friend Virus Anemia-inducing (FVA) cells, scanning electron microscopy (SEM), immunofluorescence combined with laser scanning confocal microscopy (LSCM), the FVA cells induced by Erythropoietin (EPO) for were used for: 1) to study the shape and proportion of cells at different differentiating stages, enucleation process, the formation of blood islands, and engulfment of nuclei by macrophages in real time, 2) to quantitatively analyze erythroid cell surface markers including CD71 and Ter119, and cytoskeletal-associated proteins (stathmin, septin8 and RBBP4). The results of real-time monitoring of enucleation indicated that it took about 7 to 8 hours to extrude the nuclei from karyopyknosis (polychromatic erythroblasts). It further showed .. View More»
  DOI: 10.4172/2329-6917.1000188

  Abstract PDF

Relevant Topics