ISSN: 2161-1068
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Yannick Kamdem Simo
Cameroon
Research Article
First Insight into Molecular Epidemiology of Tuberculosis Infection in Slaughtered Sheep Intended to Human Consumption in Cameroon: The Case of New-Bell’s Slaughterhouses
Author(s): Francioli Koro Koro, Ateugieu Guemechieu Romaric, EM Tchamba Kombou, Onana Ariane Tatiana, Yannick Kamdem Simo, JP Assam Assam, V Penlap Beng, Ngono Ngane Rosalie and Etoa Francois XavierFrancioli Koro Koro, Ateugieu Guemechieu Romaric, EM Tchamba Kombou, Onana Ariane Tatiana, Yannick Kamdem Simo, JP Assam Assam, V Penlap Beng, Ngono Ngane Rosalie and Etoa Francois Xavier
Objective: Tuberculosis (TB) remains understudied in Cameroonian sheep which are very consumed by human beings and which nevertheless live in close contact with cattle in which TB remains endemic. We carried for the first time a six months descriptive cross sectional study on slaughtered sheep in the major slaughter-house of Douala, from November 2013 to April 2014.Methods: About 2922 slaughtered sheep were successively inspected for tuberculosis through visual examination and palpation of carcasses, lungs, livers, hearts, intestine, inner carcasses and lymph nodes. ZiehlNeelsen staining, culture on Lowenstein Jensen solid media supplemented with Pyruvate or Para Nitro Benzene (PNB) and molecular techniques (Genotype Mycobacterium CM/AS assay and Spoligotyping) were used to identify atypical mycobacteria and Mycobacterium tuberculosis Complex (M.. View More»
DOI:
10.4172/2161-1068.1000259