Journal of Clinical and Experimental Ophthalmology
Open Access

Yoshihiro Takamura

Publications

• Research Article
  Inhibitory Effect of Alpha-Crystallins on VEGF-Induced Proliferation of Retinal Microvascular Endothelial Cells
  Author(s): Shogo Arimura, Yoshihiro Takamura, Seiji Miyake, Atsushi Kojima, Shuji Sakurai, Kazuhiro Tsuruma, Hideaki Hara and Masaru Inatani Shogo Arimura, Yoshihiro Takamura, Seiji Miyake, Atsushi Kojima, Shuji Sakurai, Kazuhiro Tsuruma, Hideaki Hara and Masaru Inatani
  
  Purpose: Lens extraction surgery with ischemic retinopathy may trigger neovascularization, suggesting that lens removal alters the ocular concentrations of angiogenesis regulatory factors. This study aims to identify angiogenic or anti-angiogenic factors exhibiting altered expression in the vitreous after lens extraction in rabbits. Methods: Vitreous samples after lens extraction were analyzed by two dimensional fluorescence difference gel electrophoresis (2D-DIGE) followed by mass spectrometry to identify candidate factors indicating expression changes. Western blotting and real-time polymerase chain reaction (PCR) were performed to semi-quantify protein and gene expression changes. The identified factors were tested by assessing effects on vascular endothelial growth factor (VEGF)-induced proliferation of human retinal microvascular endotheli.. View More»
  DOI: 10.4172/2155-9570.1000715

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