Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Yutaka Unuma
Japan
Research Article
Evaluation of a Novel Automated Machine, the Auto2D, for Two-Dimensional
Gel Electrophoresis
Author(s): Yuki Tani, Takashi Tajima, Akira Kawai, Yutaka Unuma, Hideki Kinoshita and Tadashi Kondo
Yuki Tani, Takashi Tajima, Akira Kawai, Yutaka Unuma, Hideki Kinoshita and Tadashi Kondo
Two-dimensional gel electrophoresis (2D-PAGE) has been a commonly used technique for protein expression studies. Application of a fluorescent dye in 2D-PAGE, namely 2D-DIGE, has improved the performance of 2D-PAGE in terms of sensitivity, reproducibility and throughput. However, 2D-PAGE still requires a degree of skill, and is considerably time-consuming. Recently, a novel automated 2D-PAGE machine, the Auto2D, was developed for use in conventional protein expression studies. Here, we examined the performance of the Auto2D for 2D-DIGE from the viewpoint of proteome coverage, reproducibility, and throughput. We found that a single 2D image of osteosarcoma cells contained 521 protein spots. By running an identical sample, we found that the system reproducibility was high and the intensity of more than 409 protein spots was scattered within a 2-fold range of differences between experimen.. View More»
DOI:
10.4172/jpb.1000310