Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Yuyong Hou
China
Research Article
Identification of NaHCO3 Stress Responsive Proteins in Dunaliella salina
HTBS using iTRAQ-based Analysis
Author(s): Yue Zhao, Yuyong Hou, Zhiyong Liu, Shulin Chen and Fangjian Chen
Yue Zhao, Yuyong Hou, Zhiyong Liu, Shulin Chen and Fangjian Chen
Converting CO2 to HCO3- through CO32- as a carbon source for microalgae growth offers an alternative route for CO2 fixation. There, however, are concerns over algae growth inhibition caused by high HCO3- concentration. The exact mechanism of the microalgae response to NaHCO3 stress also needs further understanding. This study evaluated the effect of high HCO3- on enhancing growth of algae Dunaliella salina HTBS by identifying a series of differentially expressed proteins in responding to NaHCO3 stress using iTRAQ (isobaric tags for relative and absolute quantitation) assay. Results showed that HTBS featured with adaptability to broad HCO3- concentrations (25 to 70 g/L NaHCO3). The specific growth rate (OD750) reac.. View More»
DOI:
10.4172/jpb.1000399