Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Zeeshan Gillani
Pakistan
Research Article
Cross-Species Amino Acids Sequence Comparison and Computational Docking of Human IL-1Ra and Rat IL-1Ra on Rat Receptor
Author(s): Muhammad Sajid Hamid Akash, Kanwal Rehman, Zeeshan Gillani, Hongying Sun and Shuqing Chen
Muhammad Sajid Hamid Akash, Kanwal Rehman, Zeeshan Gillani, Hongying Sun and Shuqing Chen
Nowadays, experimental animals are being widely used to investigate therapeutic potentials of IL-1Ra. This article states cross-species comparison of amino acid sequences of IL-1Ra and computational docking of ligands (IL-1Ra_human and IL-1Ra_rat), on rat receptor (IL-1RI_rat). The purpose of study was to understand relationship between amino acid sequences, structural and binding similarities between both ligands. We utilized molecular modeling for structure determination, sequence alignment, structure refinement and protein docking of concerned proteins. We comparatively verified amino acid sequence of both ligands using Emboss Needle method, to express pairwise sequence. Further, these sequences were used to generate 3D structural model of ligands by Phyre2, that were verified by Ramachandran plot. Docking of ligands to structure of IL-1RI_rat was attempted by using AutoDock progra.. View More»
DOI:
10.4172/jpb.1000259