Journal of Agricultural Science and Food Research
Open Access

Zemede Asfaw

Publications

• Research Article
  Bioactive Chemical Constituents from the Leaf of Oreosyce africana Hook.f (Cucurbitaceae) with Mosquitocidal Activities against Adult Anopheles arabiensis, the Principal Malaria Vector in Ethiopia
  Author(s): Damtew Bekele, Habte Tekie, Zemede Asfaw and Beyene Petros Damtew Bekele, Habte Tekie, Zemede Asfaw and Beyene Petros
  
  The use of botanical insecticides can serve as an alternative to the environmentally undesirable inorganic insecticides as a means of malaria vector control. The objective of the study was to identify the biologically active compounds of Oreosyce africana (Cucurbitaceae) leaf extracts by using a bioassay-guided approach on adult mosquitoes. The dichloromethane fraction of O. africana crude methanol extract was subjected to bioassay-guided fractionation by flash column chromatography, preparative thin-layer chromatography and high performance liquid chromatography to obtain purified fractions. The purified fractions were assayed against adult Anopheles arabiensis Patton. Gas chromatography coupled to mass spectrometry was used for identification of the active chemical compounds against adult mosquitoes. The LC50 and LC90 values were determined based on probit analysis. Two purified fra.. View More»
  DOI: 10.4172/2471-2728.1000159

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