Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access
ISSN: 2375-4508
+44 1478 350008
ISSN: 2375-4508
+44 1478 350008
Zhao Jing
China
Research Article
Development of an Animal Model for Thin Endometrium Using 95% Ethanol
Author(s): Zhao Jing, Gao Hong, Li YanpingZhao Jing, Gao Hong, Li Yanping
Objective : To develop an animal model of thin endometrium, and to evaluate the histologic effect of 95%
ethanol in the uterine cavity.
Design: Experimental prospective.
Setting: Teaching hospital affiliated with Central South University, Changsha.
Patient(s): Twenty and five female adult rats.
Intervention(s): Animals were divided into three groups: control group were submitted to injection of physiological saline in uteri horn, 5 minutes group received 95% ethanol with 5 minutes of retention, 10 minutes group received 95% ethanol with 10 minutes of retention. Endometrial morphology was analyzed by hematoxylin-eosin staining (HE), and the growth of the epithelial, stromal and vascular cells were evaluated by immunohistochemistry with cytokeratin, vimentin and VEGF.
Main outcome measure(s): Histologic effects.
Result(s): Fourteen uterin.. View More»