Journal of Leukemia
Open Access

Zhongqing Wang

Publications

• Short Communication
  Detection of BRAF V600E Mutation in Langerhans Cell Histiocytosis Using High-resolution Melting Analysis in Decalcified, Paraffin-embedded Tissue
  Author(s): Bin Fu, Zhongqing Wang, Xiaolin Li, Sa A Wang and Zhuang Zuo Bin Fu, Zhongqing Wang, Xiaolin Li, Sa A Wang and Zhuang Zuo
  
  The oncogenic BRAF V600E mutation has recently been found in a substantial number of Langerhans cell histiocytosis (LCH) cases. We developed and validated a rapid and sensitive high-resolution melting (HRM) analysis?based assay to detect BRAF V600E mutation in formalin-fixed, paraffin-embedded (FFPE), decalcified bone tissues, including 32 LCH cases and four primary bone lymphoma cases. BRAF V600E mutation was found in 18 (56%) of the LCH cases. HRM results were confirmed by Sanger sequencing. Our assay is thus a rapid, sensitive, and low-cost way to detect the BRAF V600E mutation in LCH FFPE samples for clinical diagnostic use... View More»
  DOI: 10.4172/2329-6917.1000101

  Abstract PDF

Relevant Topics