Journal of Agricultural Science and Food Research
Open Access
ISSN: 2593-9173
ISSN: 2593-9173
Department of Coffee Breeding and Genetics at Jimma Agricultural Research Center, Ethiopian Institute of Agricultural Research, Ethiopia
Research Article
Progress in Biotechnological Applications to Coffee Wilt Disease
Author(s): Admikew Getaneh*
Molecular markers and biochemical compositions are vital to distinguish genotypes for specific traits and rapid means of identification of important traits. Random amplification of polymorphic DNA- polymerase chain reaction (RAPD-PCR) analysis, single-enzyme, agarose gel-based amplified fragment length polymorphism (AFLP), enzymatic digestion of the intergenic spacer (IGS) region, and inter-simple sequence repeat (ISSR) analysis and amplification result showed that there exist distinguishable Arabica and Robusta coffee wilt disease (CWD) pathogenic forms. Based on the translation elongation factor, the calmodulin gene, and the histone gene analysis; four phylogenetic species (PS) within the Gibberella xylarioides complex were identified and are consistent with the four biological species (BS) groups (BS1, BS2, BS3, and SG4). A single mating gene (MAT) gene of two different forms (alle.. View More»