Advanced Techniques in Biology & Medicine
Open Access

Department of Pharmaceutical Biotechnology, Portland Laboratory in De Montfort University in Leiceste, UK

Publications

• Research   
  Isolation of Mitochondrial RNA with 6-OHDA Treatment to Detect Cell Viability of HtrA2 KO/CHOP Genotype
  Author(s): Al-Baraa Akram El-Sayed*
  
  Mitochondrial RNAs are a class of small RNAs which can regulate gene expression through interference of translation process. A particular class of these miRNAs was found to be enriched in proximity of mitochondria and inside these organelles controlling the behavior of the mitochondria. The RNA in mitochondria is organized by special and membrane less compartments of RNA-protein complexes, called the RNA mitochondrial granules, which are abbreviated into MRGs. Most methods used to isolate mitochondria depends on differential centrifugation, a two-step centrifugation carried out at low speed to remove intact cells, cell and tissue debris as well as nuclei from whole cell extracts followed by high speed centrifugation to concentrate mitochondria and separate mitochondria from other cellular organelles. Molecular quality control marker Hsp60 and organ-cellular qu.. View More»
  DOI: 10.35248/2167-1044.22.10.380

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