Journal of Proteomics & Bioinformatics
Open Access

Department of Medicine, University of Geneva, Geneva, Switzerland

Publications

• Perspective   
  Tandem Mass Tags (TMT) as an Important Isobaric Labelling Method for Protein Analysis
  Author(s): Alexander Dayon*
  
  Tandem Mass Tags (TMT) are chemical tags that facilitate sample multiplexing for Mass Spectrometry (MS) based quantification and identification of biopolymers such as proteins, peptides, and nucleic acids. TMT belongs to a family of reagents called isobaric mass tags. This is a set of molecules with the same mass, but producing reporter ions with different masses after fragmentation. The relative proportions of measured reporter ions represent the relative abundance of the labelled molecule, but ion suppression adversely affects accuracy. MALDI-TOF-based protein fingerprinting is used to digest the sample with a specific protease (usually trypsin) and acquire an MS spectrum that produces the masses of all peptides (or MH+). These qualities are used as fingerprints for searching proteins in the database and matching them with their measured masses. Protein tags are convenient and usefu.. View More»
  DOI: 10.35248/0974-276X.23.16.638

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