Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Nevada Institute of Personalized Medicine, University of Nevada, Las Vegas, Nevada 89054-4004, United States of America
Research Article
New Minimotifs and their Functions in the C-Terminome
Author(s): Surbhi Sharma, Nemanja Novakovic, Zachary Thomas FitzHugh, Alexandria Bragg, Stephen Brooks, Xiaogang Wu and Martin R. Schiller*
Several protein domains and receptors recognize minimotifs on the short carboxyl termini of proteins and
control protein clustering, trafficking, and posttranslational modifications. These functional C-terminal
minimotifs are found on approximately 17% of proteins in the human proteome, with 83% of unknown
functional significance. We tested a bioinformatic/proteomic approach to systematically identify new C-terminal
minimotif functions. We selected 30 putative consensus C-terminal minimotifs based on their fold-enrichment
and sequence complexity. These 30 consensus minimotifs had instances on 16% of the C-termini in the
human proteome. Binding partners for a representative instance for each consensus C-terminal minimotif were
identified by affinity purification liquid chromatography-tandem mass spectrometry. We validated the approach
with a PDZ binding C-termin.. View More»