Journal of Infectious Diseases & Preventive Medicine
Open Access
ISSN: 2329-8731
+44 1300 500008
ISSN: 2329-8731
+44 1300 500008
Department of Biotechnology, Viroclinics Biosciences, Rotterdam, Netherlands
Research Article
Mechanism of Antiviral Activity of Nitazoxanide: Effect on Adenosine Triphosphate in Influenza-Virus Infected Madin Darby Canine Kidney
Cells
Author(s): Jean-François Rossignol*, Aloys SL Tijsma and Carel A van Baalen
Background: Nitazoxanide (NTZ), a broad-spectrum antiviral undergoing clinical development for treating influenza, COVID-19 and other viral respiratory illnesses, is a mild uncoupler of oxidative phosphorylation.
Methods: We evaluated the effect of Tizoxanide (TIZ), the active circulating metabolite of NTZ, on Adenosine Triphosphate (ATP) and cell viability in Madin-Darby Canine Kidney (MDCK) cells and in MDCK cells infected with influenza A and B viruses.
Results: TIZ decreased cellular ATP in a dose-dependent manner in MDCK cells and in MDCK cells infected with influenza A and B viruses. Maximum inhibition of ATP in influenza-infected or uninfected MDCK cells reached up to 45% after 6 and 24 hours of exposure to 100 μM TIZ. The decrease in cellular ATP did not affect cell viability and was reversible after eli.. View More»
DOI:
10.35841/2329-8731.22.10.262