Chemotherapy: Open Access
Open Access
ISSN: 2167-7700
ISSN: 2167-7700
Laboratório de Bioquímica e Biologia Molecular Aplicada, Departamento de Genéti, Universidade Federal de São Carlos, São Carlos, Brazil
Research Article
Antitumor Effect of Il-2 and TRAIL Expressed by Salmonella: An Immunotherapeutic Proposal against Bladder Cancer In Vitro and In Vivo by iNOS, Caspase Activation, Immune System Modulation and Tumor Regression
Author(s): Bruna Dias de Lima Fragelli*, Joice Margareth de Almeida Rodolpho, Krissia Franco de Godoy, Luciana Camillo, Cynthia Aparecida de Castro, Patricia Brassolatti, Ricardo Carneiro Borra, Adilson José da Silva, André Vessoni Alexandrin, Maria Teresa Marques Novo-Mansur, Camila Tita Nogueira and Fernanda de Freitas Anibal
IL-2 and TRAIL are therapeutic agents known for their antitumor role, but they can be cleared quickly by the body or be cytotoxic, compromising their function. To reverse this impasse, the synthesis of these agents by live bacteria directly in the tumor is a viable approach, due to bacteria’s preference to colonize tumor tissue. It happens because of the high nutrient grid in this microenvironment and possible escape from the immune system since solid tumors are ischemic and have regions of hypoxia. In this context, attenuated Salmonella represents a promising live vector for delivery of antitumor molecules. The present study investigated the effects of IL-2, TRAIL and mixture of proteins expressed by recombinant Salmonella, strain SL3261, with unique and innovative construction with IL-2 and TRAIL proteins, on bladder cancer cells and in mice od C57BL/6. Human.. View More»
DOI:
10.35248/2167-7700.23.11.171