Enzyme Engineering
Open Access
ISSN: 2329-6674
ISSN: 2329-6674
Department of Biochemistry, University of Calabar, Calabar, Nigeria
Research Article
Effect of Sudan II Adulteration of Palm Oil on the Serum Enzyme, Bilirubin Concentration and Renal Function Biomarkers of Albino Wistar Rats
Author(s): Henry E Peters*, Aniekan S Henshaw, Christine A Ikpeme and Ima-obong Williams
Aim: This study investigated the impact of Sudan II adulteration of palm oil on serum enzymes (ALT, AST, ALP), bilirubin and renal function biomarkers (creatinine, urea) of albino wistar rats.
Methods: A total of sixty (60) 750 mL bottles of red palm oil were purchased from random markets in Nigeria. Sixty (60) male albino rats weighing 150-180 g were divided into 5 groups of 12 rats each. Group 1 served as normal control. Groups 2 to 5 were fed 90% rat chow supplemented with 10% red palm oil. The Sudan II dyes were co- administered with the red palm oil with the diet (rat chow) to provide levels of 0.025% (PO/0.025) (group 3), 0.03% (PO/0.03) (group 4) and 0.04% (PO/0.04) (group 5) for a period of 30 days (short term) and 90 days (long term). Animals were sacrificed and blood was collected via cardiac puncture for biochemical analy.. View More»
DOI:
10.35841/2329-6674.22.11.1000176