Journal of Chromatography & Separation Techniques
Open Access

Department of Analytical Chemistry, Dr. Reddy’s Laboratories Ltd., Banjara Hills, Hyderabad, 500034, Telangana, India

Biography

Ankit C is currently working in the Department of Analytical Chemistry for the Dr. Reddy’s Laboratories Ltd located in India, International experience includes various programs, contributions and participation in different countries for diverse fields of study.

Publications

• Research Article   
  A Rapid LC-MS/MS Method for Quantification of Acetaminophen in Human Whole Blood
  Author(s): Divya Vilas K, Sandeep Reddy C, Prashanthi R and Ankit C*
  
  Acetaminophen (paracetamol, N-(4-hydroxyphenyl) acetamide) is one of the most prescribed drugs for the pain management, minor aches, and treatment of fever. Quantification of acetaminophen in pre-term and term neonates and small children requires the availability of highly sensitive assays in small volume blood samples. We developed and validated an LC-MS/MS assay for the quantification of acetaminophen in human whole blood. The drug molecule was extracted from whole blood samples followed by protein precipitation extraction technique followed by LC-MS/MS analysis. An excellent analyte separation was achieved by using a phenomenex Gemini® C18 column (50× 3.0 mm, 3 μm) using mobile phase A-Water:Formic Acid (100:0.1) and Acetonitrile:Formic acid (100:0.1) as mobile phase B. With 3.40 minutes as analytical run time, this analytical method demonstrated linearity between 50.0.. View More»
  DOI: 10.35248/2157-7064.20.11.432

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