Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Center for Translational Immunology, University Medical Center Utrecht (UMCU), Utrecht, The Netherlands
Original Research Article
Pluripotency Factor PBX1 Predicts Treatment Efficacy in Rheumatoid Arthritis
Author(s): Karin M.E. Andersson, Eric Malmhäll-Bah, Nina Y.Oparina, Weiyang Tao, Aridaman Pandit, Malin C. Erlandsson, Venkataragavan Chandrasekaran, Sofia T. Silfverswärd, Rille Pullerits and Maria I. Bokarewa*
Objective: We study molecular mechanisms behind T cell reconstitution in Rheumatoid Arthritis (RA) by asking if PBX1, a transcription factor that maintains stem cell pluripotency, predicts antirheumatic treatment response.
Methods: Whole-genome transcriptomics by RNA sequencing was done in CD4+T cells from 87 RA patients with low, and from 78 RA patients with high disease activity. Treatment outcomes in PBX1hi and PBX1lo groups were compared. PBX1-associated phenotype and biological processes were identified by clustering of the genes differentially expressed in PBX1hiCD4+ cells. PBX1hi clusters in thymus were identified by a single cell-based analysis. PBX1 transcriptional targets among DEGs were predicted by the integrative analysis of DNA motif, chromatin immunoprecipitatio.. View More»
DOI:
10.35248/2155-9899.22.13.668