Journal of Plant Biochemistry & Physiology
Open Access
ISSN: 2329-9029
+44 1478 350008
ISSN: 2329-9029
+44 1478 350008
Coffee Research Institute, KALRO, Kenya
Research Article
Effects of Genotype and Plant Growth Regulators on Callus Induction in Leaf Cultures of Coffea arabica L. F1 Hybrid
Author(s): Irene WM*, Alumiro HL, Asava KK, Agwanda CO and Anami SE
Access to planting materials is one of the main challenges constraining the widespread adoption of the disease resistant Coffea arabica L. F1 hybrid variety Ruiru 11 in Kenya. Production of the planting materials for the variety relies on several cost-intensive methods including hand pollination for hybrid seed production and vegetative propagation through cuttings. These seed production methods are inefficient and costly and rely heavily on the weather conditions. Production and supply of planting materials for the commodity is therefore unable to meet the annual demand for the variety. In an attempt to bridge the gap between the supply and demand, tissue culture technique has been deployed at the Coffee Research Institute in Kenya. This however requires empirical tests to optimise in vitro mass propagation protocols for hybrid coffee varieties. The current study in.. View More»