Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
ISSN: 2155-9600
Department of Medical Research, National Institutes of Health, Selangor, Malaysia
Research Article
Determination, Fractionation and Quantification of Vitamin D in Pleurotus ostreatus (Oyster Mushroom) Using Recycling Preparative High Performance Liquid Chromatography (HPLC)
Author(s): Aswir Abd Rashed*, Rathi Devi Nair Gunasegavan and Shahrul Razid Sarbini
Recent studies have demonstrated that vitamin D insufficiency is common in tropical countries. Vitamin D is a fat-soluble vitamin that is obtained by sunlight exposure, diet, or supplements and it is an important factor influencing the health status of individuals. Vitamin D is naturally present in the mushroom and once exposed to a source of Ultraviolet (UV) radiation, can generate nutritionally relevant amounts of vitamin D. Hence, the purpose of this study is to determine, fractionalised and quantify the amount of vitamin D in the oyster mushroom (Pleurotus ostreatus). An amount of fine powder mushroom was weighed and incubated with 20 ml hexane and 50% potassium hydroxide mixture for 10 minutes at 37°C. The mixture solvent was then centrifuged for 45 minutes at 2000 rpm before the supernatant was stored at -20°C for a week until a white precipitate was formed. Lat.. View More»
DOI:
10.35248/2155-9600.23.13.018